160 gd conjugated mouse anti human cd14  (fluidigm)

Journal: Molecular Systems Biology

Article Title: Systems approach identifies monocyte imbalance in symptomatic and asymptomatic P. vivax malaria

doi: 10.1038/s44320-025-00135-z

Figure Lengend Snippet: Peripheral blood mononuclear cells from P. vivax symptomatic ( n = 8), asymptomatic ( n = 8), and healthy controls ( n = 8) were stained with a panel of metal-labeled antibodies and analyzed CyTOF. ( A ) Manual gating was used to select for CD14 + monocytes. ( B ) vi-SNE analysis was performed on pooled monocytes for each group and the cell surface expression was illustrated by their density.

Article Snippet: 160 Gd-conjugated mouse anti-human CD14 , Fluidigm , Clone RMO52, Cat.# 3160006B.

Techniques: Staining, Labeling, Expressing

Journal: Molecular Systems Biology

Article Title: Systems approach identifies monocyte imbalance in symptomatic and asymptomatic P. vivax malaria

doi: 10.1038/s44320-025-00135-z

Figure Lengend Snippet: ( A – F ) PBMCs from P. vivax symptomatic ( n = 8) and asymptomatic ( n = 8) infected individuals as well as healthy immune controls ( n = 8) were stained with a panel of metal-labeled antibodies and analyzed by CyTOF. ( A ) t-SNE analysis was performed on CD14 + CD3 - CD29 - monocytes. Plots display cell density and represent the pooled data for each group. ( B ) Projection of FlowSOM clusters on a t-SNE plot. ( C ) Heatmaps show the median marker expression for each FlowSOM cluster. ( D – F ) Unsupervised identification of differentially abundant populations between clinical groups was performed using CITRUS (FDR < 5%) between clinical groups. Panels on the top left or each panel show differentially abundant populations on a t-SNE overlay identified as follows: significantly more abundant in symptomatic individuals compared to healthy immune controls ( D ), significantly more abundant in symptomatic and asymptomatic individuals compared to healthy immune controls ( E ) and significantly depleted in symptomatic and asymptomatic individuals compared to healthy immune controls ( F ). vi-SNE plots on the top right-hand side or each top panel depict relevant marker expression on t-SNE overlays. The lower left panels show the frequency of differentially abundant cell populations identified by CITRUS. Boxes represent the 25th to 75th percentile, whiskers show the range (minimum to maximum), and lines represent the median of 8 symptomatic, 8 asymptomatic, and 8 healthy immune control biological replicates. The lower right panels illustrate marker expression in differentially abundant populations (pink histograms), relative to background expression (lilac histograms).

Article Snippet: 160 Gd-conjugated mouse anti-human CD14 , Fluidigm , Clone RMO52, Cat.# 3160006B.

Techniques: Infection, Staining, Labeling, Marker, Expressing, Control

Journal: Cell

Article Title: NKG2A blockade potentiates CD8 T-cell immunity induced by cancer vaccines

doi: 10.1016/j.cell.2018.10.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD14 – 160 Gd (M5E2) , Fluidigm , Prod#: 3151009B.

Techniques: Blocking Assay, Purification, Virus, Recombinant, Staining, Transfection, Cell Stimulation, Adjuvant, Mutagenesis, Transgenic Assay, Marker, Sequencing, Expressing, Plasmid Preparation, Software, CRISPR